Molecular Virology
The Broadbent lab aims to advance our understanding of the molecular basis underpinning the replication and pathogenesis of avian viruses, to better prevent and control diseases of significance to the poultry industry, and to public health. Current projects are focused on viruses with segmented RNA genomes, including: avian influenza virus (AIV), infectious bursal disease virus (IBDV), and avian reovirus (ARV).
The lab uses these viruses as tools to:
1. Improve the control of animal viruses- Improve the efficacy of current vaccines, improve the design of future vaccines, identify gene targets for engineering more resistant animals.
2. Model pathogenesis and immunosuppression- Identify host and viral factors that influence the outcome of disease, determine how immunosuppression in livestock impacts upon the pathogenesis, evolution & transmission of potentially zoonotic infectious diseases, define immunosuppression at mucosal surfaces, characterize the recovery from immunosuppression.
3. Determine viral replication mechanisms- Define the nature of virus replication complexes, characterize the host-cell antiviral response, determine the molecular mechanisms underpinning reassortment
4. Control other diseases in animals and humans- Determine the utility of viruses as vaccine vectors and in oncolytic viral therapy
We use a blend of in vitro cell culture, primary tissues and cells, and in vivo studies, and employ molecular biology, immunological, and bio- imaging techniques in the lab. Please get in touch if you are interested in opportunities to join the team.
More information
Education and Experience
2021- , Assistant Professor, Virology, Department of Animal and Avian Sciences, University of Maryland, College Park, MD, USA
2020-2021, Teaching Fellow, Immunology & Virology, School of Veterinary Medicine, University of Surrey, UK
2019-2021, Group Leader, The Pirbright Institute, UK
2014-2019, Research Fellow, The Pirbright Institute, UK
2010-2014, Postdoctoral Visiting Fellow, Virology, National Institutes of Health (NIH), Bethesda, MD, USA
2006-2010, PhD, Microbiology & Immunology, Imperial College London, UK
2005-2006, MSc, London School of Hygiene and Tropical Medicine, UK
2002-2005, VetMB, Veterinary Medicine, University of Cambridge, UK
1999-2002, MA, Pathology, University of Cambridge, UK
Past and Present members of the team
Present Members
Postdoctoral Researchers
Dr Sofia Egana-Labrin, Postdoctoral Research Associate, 2022-present
Graduate students
Andrew Brodrick, PhD student, 2021- present
Undergraduate and placement students
Declan Kehlbeck, undergraduate student, ANSC, 2022-present
Megan Liu, undergraduate student, ANSC, 2023- present
Past members
Undergraduate and placement students
Alisha van Eck, undergraduate student, ANSC, 2021-2022
Shelley Wang, summer placement student, UMB, 2022
Eunice Ok, undergraduate student, SPHL, 2023
Amber Rayford, undergraduate student, CBMG, 2023
Publications
2023: Egana-Labrin S, Broadbent AJ. Avian Reovirus: a furious and fast evolving pathogen. Journal of Medical Microbiology, 2023, Oct 6 2023;72:001761. doi:10.1099/jmm.0.001761
2023: Nazki S, Reddy VRAP, Kamble N, Sadeyen J, Iqbal M, Behboudi S, Shelton H, Broadbent AJ. CD4+TGFβ+ cells infiltrated the bursa of Fabricius following IBDV infection, and correlated with delayed viral clearance, but did not correlate with disease severity, or immunosuppression. Frontiers in Immunology, 12:1197746. doi: 10.3389/fimmu.2023.1197746.
2023: Francois S, Nazki S, Vickers SH, Fournie G, Perrins CM, Broadbent AJ, Pybus OG, Hill SC. Genetic diversity, recombination and cross-species transmission of a waterbird gammacoronavirus in the wild. Journal of General Virology, 2023, Aug 104(8). doi: 10.1099/jgv.0.001883.
2023: Brodrick AJ, Broadbent AJ. The formation and function of Birnaviridae virus factories. International Journal of Molecular Sciences, 2023, May 9;(24):8471. doi:10.3390/ijms24108471.
2023: Reddy VRAP, Nazki S, Asfor A, Broadbent AJ. An Infectious Bursal Disease Virus (IBDV) Reverse Genetics Rescue System and Neutralization Assay in Chicken B cells. Current Protocols, 2023, Jan 3(1):e369. doi: 10.1002/cpz1.639.
2022: Asfor AS, Reddy VRAP, Nazki S, Urbaniec J, Brodrick AJ, Broadbent AJ. Modeling Infectious Bursal Disease Virus (IBDV) Antigenic Drift In Vitro. Viruses, 2022, Dec 31;15(01):130. doi: 10.3390/v15010130.
2022: Reddy VRAP, Nazki S, Brodrick AJ, Asfor A, Urbaniec J, Morris Y, Broadbent AJ. Evaluating the breadth of neutralizing antibody responses elicited by infectious bursal disease virus (IBDV) genogroup A1 strains using a novel chicken B-cell rescue system and neutralization assay. Journal of Virology, 2022, Sep 7;e0125522.doi: 10.1128/jvi.01255-22
2022: Reddy VRAP, Campbell EA, Wells J, Simpson J, Nazki S, Hawes PC, Broadbent AJ. Birnaviridae virus factories show features of liquid-liquid phase separation, and are distinct from paracrystalline arrays of virions observed by electron microscopy. Journal of Virology, 2022, Feb 9;jvi0202421. doi: 10.1128/jvi.02024-21.
2021: Asfor A, Nazki S, Reddy VRAP, Campbell E, Dulwich KL, Giotis ES, Skinner MA, Broadbent AJ. Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo. Viruses, 2021, 13(5), 933; doi: 10.3390/v13050933
2020: Dulwich KL, Gray A, Asfor A, Giotis S, Skinner M, Broadbent AJ. The stronger downregulation of in vitro and in vivo innate antiviral responses by a very virulent strain of infectious bursal disease virus (IBDV), compared to a classical strain, is mediated, in part, by the VP4 protein. Frontiers in Cellular and Infection Microbiology, 2020, June 9, 10.315. doi: 10.3389/fcimb.2020.00315
2020: Campbell E, Gray A, Skinner M, Jennifer Simpson, Pippa Hawes, Broadbent AJ. Discrete virus factories form in the cytoplasm of cells co-infected with two strains of the segmented dsRNA virus, infectious bursal disease virus (IBDV), that subsequently coalesce. Journal of Virology, 2020, Jun 16; 94 (13), e-02107-19, doi: 10.1128/JVI.02107-19.
2018: Dulwich KL, Asfor AS, Gray AG, Nair V, Broadbent AJ. An ex vivo chicken primary bursal-cell culture model to study infectious bursal disease virus pathogenesis. Journal of Visualized Experiments, 2018; Oct 4; (140); doi: 10.3791/58489
2018: Sadigh Y, Powers C, Spiro S, Pedrera M., Broadbent A, Nair V. Gallid hepesvirus 3 SB-1 strain as a recombinant viral vector for poultry vaccination. NPJ Vaccines, 2018; May 28; 3:21; doi: 10.1038/s41541-018-0056-6
2018: Soubies S, Courtillon C, Abed M, Amelot M, Keita A, Broadbent A, Härtle S, Kaspers B, Eterradossi N. Propagation and titration of infectious bursal disease virus, including non-cell- culture-adapted strains, using ex vivo-simulated chicken bursal cells. Avian Pathology, 2017; Jan; doi: 10.1080/03079457.2017.1393044
2017: Dulwich KL, Giotis ES, Gray A, Nair V, Skinner M, Broadbent AJ. Differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV). Journal of General Virology, 2017; 98:2918-2930; doi:10.1099/jgv.0.000979
2017: Ciccone NA, Smith LP, Mwangi W, Boyd A, Broadbent AJ, Smith AL, Nair V. Early pathogenesis during infectious bursal disease in susceptible chickens is associated with changes in B cell genomic methylation and loss of genome integrity. Developmental and Comparative Immunology, 2017 Mar; doi: 10.1016/j.dci.2017.03.014.
2017: Houser KV, Broadbent AJ, Gretebeck L, Vogel L, Lamirande EW, Sutton T, Bock KW, Minai M, Orandle M, Moore IN, Subbarao K. Enhanced inflammation in New Zealand white rabbits when MERS-CoV reinfection occurs in the absence of neutralizing antibody. PLoS Pathogens, 2017 Aug 17; 13(8):e1006565; doi: 10.1371/journal.ppat.1006565
2016: Broadbent AJ, Santos CP, Anafu A, Wimmer E, Mueller S, Subbarao K. Evaluation of the attenuation, immunogenicity, and efficacy of a live virus vaccine generated by codon-pair bias de-optimization of the 2009 pandemic H1N1 influenza virus in ferrets. Vaccine, 2016 Jan 20; 3(4): 563-570; doi: 10.1016/j.vaccine.2015.11.054.
2015: Broadbent AJ, Santos CP, Paskel M, Matsuoka Y, Lu J, Chen Z, Jin H, Subbarao K. Replication of live attenuated cold-adapted H2N2 influenza virus vaccine candidates in non human primates. Vaccine, 2015 Jan 1; 33(1): 193-200; doi: 10.1016/j.vaccine.2014.10.065.
2014: Broadbent AJ, Santos CP, Godbout R, Subbarao K. The Temperature-Sensitive and Attenuation Phenotypes Conferred by Mutations in the Influenza Virus PB2, PB1, and NP Genes Are Influenced by the Species of Origin of the PB2 Gene in Reassortant Viruses Derived from Influenza A/California/07/2009 and A/WSN/33 Viruses. Journal of Virology, 2014 Nov 1; 88(21): 12339-12347; doi: 10.1128/JVI.02142-14.
2014: Lakdawala SS, Wu Y, Wawrzusin P, Kabat J, Broadbent AJ, Lamirande EW, Fodor E, Altan-Bonnet N, Shroff H, Subbarao K. Influenza A virus assembly intermediates fuse in the cytoplasm. PLoS Pathogens, 2014 Mar 6;10(3):e1003971; doi:10.1371/journal.ppat.1003971.
News Articles
ANSC’s Dr. Andrew Broadbent Elected as ASV Veterinary Virology Councilor | Animal Sciences (umd.edu)
https://ansc.live.seed.umd.edu/news/ansc-undergraduate-declan-kehlbeck-awarded-scholarships-work-related-avian-viruses
https://agnr.umd.edu/momentum-magazine/summer-2023/preventing-poultry-pandemic
Teaching
ANSC 101, "Principles of Animal Science", Co-coordinator and instructor (Spring 2023), guest lecturer (Fall 2023)
ANSC 340, "Health Management of Animal Populations" (a course on animal infectious diseases and biosecurity), coordinator and instructor (Spring 2022-present)
ANSC 440 / 688J, "Zoonotic Diseases and Control", co-instructor (Spring 2024)
ANSC 460 / 688I, "Comparative Vertebrate Immunology", guest lecturer, (Fall 2021-present)
VMSC 670, "Molecular Epidemiology of Infectious Diseases", guest lecturer (Spring 2022- present)
Links
Threads and the platform formerly known as twitter: @DrAndyBroadbent
LinkedIN: https://www.linkedin.com/in/andrew-broadbent-4414b513/
Google Scholar: https://scholar.google.co.uk/citations?user=YAzMzXQAAAAJ&hl=en
